杏鮑菇蛋白提取方法的研究

1、杏鮑菇蛋白提取方法的研究摘要摘要以杏鮑菇副產物為原料通過干燥、粉碎、堿提、酸沉、回調、凍干等工藝提取杏鮑菇蛋白質。研究了pH值、固液比、提取溫度和提取時間等單因素對蛋白質提取率的影響并經正交試驗優(yōu)化提取工藝條件。結果表明：杏鮑菇蛋白質適宜提取工藝條件為pH值11.0、固液比1：40（gmL）、提取時間40min、杏鮑菇蛋白質提取率為（42.371.63）%、純度（63.40.87）%。關鍵詞：杏鮑菇；蛋白質；破提AbstractUsin

2、gPleurotuseryngiibyproductastherawmaterialbythetechnologyofdryingcrushingalkaliextractionacidprecipitationcallbackfreezedryingthePleurotuseryngiiproteinwasextracted.TheeffectsofpHvaluesolidliquidratioextractiontemperatur

3、eextractiontimeonproteinextractionratewerestudied.thethogonaldesignwasusedtooptimizetheextractionconditions.TheresultshowedthattheoptimumconditionofextractionprocessfPleurotuseryngiiproteinwasatpH11.0solidliquidratio1:40

4、(gmL)extractiontime40min.UnderthisconditiontheextractionrateofPleurotuseryngiiproteinwas(42.371.63)%theproteincontentwas(63.40.87)%.Keywds:Pleurotuseryngiproteinalkaliextraction目錄前言.......................................

5、...............11.材料與方法..............................................11.1.原料與試劑..........................................11.2.儀器與設備..........................................11.3.試驗方法......................................

6、......22.結果與分析..............................................32.1.牛血清白蛋白標準曲線................................32.2.單因素試驗..........................................42.3.正交試驗結果........................................63.總結.....