大豆疫霉根腐病病原菌生物學特性及抗源篩選研究.pdf

1、東北農業(yè)大學碩士學位論文大豆疫霉根腐病病原菌生物學特性及抗源篩選研究姓名：呂慧穎申請學位級別：碩士專業(yè)：作物遺傳育種指導教師：楊慶凱2000.5.1查苧奎些查蘭盔蘭筌曼堡塑塑墮曼里蘭塑蘭絲絲墨苧翌絲壟翌塞：竺!蘭：星StudyOilBiologicalCharacteristicsofPhytophthorasojaeandScreeningofGermplasmforResistancetothepathogenabstractPhy

2、tophthorarootrotofsoybeancausedbyPhytophthorasojaeisoneofthemostdangeroussoybeandiseaseThediseasewasfirstfoundinHeilon西iangprovinceofChinain199Iandthenithasbeella辯dousdiseasewhichhasseverelatentdamageofsoybeanproductioni

3、nHeilongjiangProvinceThisstudyservedasabasicresearchforresistancebreedingastudyoffactorswhichinfleuncedthegrowthofmyceliumofPhytophthorasojaeanditsz00sporesproducaonwascarriedoutbasedonR25ofPsojaeByusingR25andH4ofthepath

4、ogen，956varieties(orlines)fromHeilongiiang，JilinLiaoningProvincewereevaluatedforscreeningresistantgermplasm，whichcarlprovideresistantgermplasmandparentsfordisease—resistantbreedingprogrammeaswell∞providebasicmamfialsforb

5、asicresearchofresistantgeneticsresistantmachnismetcTheresultsofthisstudyshowedthatsuitabletemperatureformyceliumgrowthrangedfrom240Cto320Candtheoptimumtemperaturewere280cThepathogencouldgrowinmediumwithpHvalmrangingfrom2

6、to9butacidconditionwasfavourabletothegrowthofpathogenwhichdevelopedmuchfastwhenpHvaluewasfrom4to5Themyceliumgrewfasterwhileitwasincubatedinthedark111epathogenwasnotparticularaboutcarbonsousesitcouldutilizebothmonosacchar

7、ideandpolysaccharideTheoptimumnitrogensourceswerebeeftypetoneandasparagine，itcouldntutilizeammoniumm訂ogenverywellUnder250Cthemyceliumof8dayswereincubatedinthedistilledwaterfor72hours，suspicionsolutionofzoosporewithhighco

8、ncentrationcouldbeproducedYoungmyceliumproducedmorezoosporesthantheold，andacidconditionwasfavourabletotheproducaonofzoosporeTempemturewasanimportantfactorwhichinfluencedtheproductionofzoospore，buttherewasnozoosporerrcedu

9、cedunder00C、350C日∞appropriatetemperaturefortheproductionofzoosporerangedfrom200Cto300Candtheoptimumtemperaturewas250CThesoybeangermplasmfromthethreeNortheastProvinceswereevaluatedforresistance幻Psojae23varieties(orlines)，