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1、西北大學(xué)碩士學(xué)位論文中藥遠志遺傳多樣性的ISSR分析姓名:李佳申請學(xué)位級別:碩士專業(yè):植物學(xué)指導(dǎo)教師:趙桂仿20100603ISSRanalysisonpopulationgeneticdiversityofyuanzhiAbstractInthepresentstudyISSR(intersimplesequencerepeat)molecularmarkerswereemployedtoinvestigatethegeneticdi
2、versitywithinandamongwildpopulationsofPolygalatenuifoliaandPsibiricaandcultivatedpopulationsofPtenuifoliaTheanalysesof25wildpopulationsofthetwopolygalasshowed:(1)BoththeUPGMAclusteranalysisandthePCoAanalysisdisplayedacle
3、arseparationofthetwospecies;(2)ManyindicatorsfordiversitysuchasPPBdisplayedthatthegeneticdiversityofthetwospeciesWasrelativelyhigh;(3)Atlargegeographicalscalesthegeneticdistancesbetweenthetwospecies’populationsweresignif
4、icantlypositivelycorrelatedtothecorrespondinggeographicaldistances,whichWasconsistent、^,inltheisolation—bydistancemodelAccordingtotheaboveresultssomeimplicationsfortheconservationofthetwopolygalaswerediscussed:(1)Findpop
5、ulationsofthetwospeciesinlargesizewithinnaturereservescurrentlyestablishedandmake/nsituconservation;(2)GrowmoreherbsasthesubstitutionforwildonesTheanalysesof5wildand5cultivatedpopulationsofPtenuifoliafromthemainproductio
6、nareasshowed:(1)BoththeUPGMAclusteranalysisandthePCoAanalysisdisplayedthatthewildpopulationandthecultivatedpopulationfromthesamegeographicalareawouldbeclusteredfirst,indicatingthatcultivatedpopulationsisderiveddirectlyfr
7、omnearwildones;(2)Atsmallgeographicalscalesthegeneticdistancesbetweenpopulationsofthespecieshavenosignificantlypositiverelationships、航ththecorrespondinggeographicaldistances;(3)ThegeneticdiversityofcultivatedpopulationsW
8、aslowerthanthatofwildones,whichwascausedbytheextensivecultivationandbiologicalcharactersofthespeciesAccordingtotheaboveresultsitWassuggestedthatseedbreedingofthecultivatedpolygalashouldbedeveloped,andtherelationshipbetwe
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