紫花苜蓿再生體系的建立及根癌農桿菌介導聚磷基因ppk的遺傳轉化.pdf

1、甘肅農業(yè)大學碩士學位論文紫花苜蓿再生體系的建立及根癌農桿菌介導聚磷基因ppk的遺傳轉化姓名：施莉娟申請學位級別：碩士專業(yè)：草業(yè)科學指導教師：蒲小鵬許雷20100601甘肅農業(yè)大學2010屆碩士學位論文AbstractIntheworldwide，theeffectiveutilizationofphosphorusisgenerallylowphosphorusdeficiencyisaseriousrestrictionofforag

2、eproductionqualityandtheecological，economicthestudyseekstotransfergeneppkintothealfalfaphosphorusplantalfalfagrowingareasteducethesoilphosphatefertilizerapplication，increasetheeffectiveutilizationofphosphorus，whileatthej

3、unctionoffarmlandandwatertheestablishmentofbufferzoneofalfalfatocontroleutrophicationBasedontwovarietiesalfalfatoestablishandoptimizetheregenerationsystem，comparethebestreceptorandtheirgenetictransmissionofrecyclingsyste

4、m，usingAgrobacterium—mediatedphosphorusalfalfaGenetictransformationofgenesppkinitiallyreceivedKanresistantplantsthemajorfindingsareasfollowing：1RegenerationsystemsofXinjiangdayealfalfatheidealexperimentsterilizationmetho

5、dsisRinsingtheseedsunderrunningwaterafteranhour，thentreatment謝th70％alcohol50seconds，then10％sodiumhypochloriteshocktreatment22min；ThemostappropriateexplantsofXinjiangdayealfalfaistheleavesofnovaccineafter4weeks；themostsui

6、tablemediumforcallusinductionis：MS24D(20mg／L)KT(O5mg／L)；themostsuitablemediumforshootdifferentiationis：MS6BA(o5mg／L)NAA(005mg／L)2BaodingalfalfaregenerationsysteminduceddifferentiationofthemostsuitableexplantsofBaodingalf

7、alfaishypocotygerminationforsixdays；thebestmediumforcallusinductionphaseis：SH2，4D(20rag／L)KT(005mg／L)；Thebestbuddifferentiationmediumis：SHKT(04mg／L)NAA(03mg／L)3IdealrecipientfortransformationRegenerationfiequcncyofXinjia

8、ngdayealfalfaisis15676％，betterthanBaoding(11703)SoXinjiangdayealfalfaistheidealtransformation4AgrobacteriummediatedgenetransformationofantidroughtgeneUsingtheAsepticseedlingsleafthattraining4weeksasexplants，agrobacterium